Journal: eLife

Article Title: HCN2 channels in the ventral tegmental area regulate behavioral responses to chronic stress

doi: 10.7554/eLife.32420

Figure Lengend Snippet:

Article Snippet: To reveal labeled cells using the juxtacellular method, VTA sections were first incubated with Texas Red avidin D (1:100; Vector Laboratories) to retrieve the labeled cell bodies.

Techniques: shRNA, Plasmid Preparation, Avidin-Biotin Assay, Software

Journal:

Article Title: Role of UEV-1, an Inactive Variant of the E2 UbiquitinConjugating Enzymes, in In Vitro Differentiation and Cell Cycle Behavior of HT-29-M6 Intestinal Mucosecretory Cells

doi:

Figure Lengend Snippet: Chromosomal assignment of HsUEV-1 by FISH analysis. Human metaphase chromosomes were probed with two biotinylated independent PAC clones (yellow, 44c17; red, 152g20). Both probes yielded signals that colocalized on chromosome 20q13.2 (arrows and paired green spots).

Article Snippet: Signals were amplified once with biotinylated anti-avidin D. Two independent PAC probes were labeled with biotin 16-dUTP and digoxigenin 11-dUTP, respectively, as described above, and were visualized with avidin-FITC and anti-digoxigenin-tetramethyl rhodamine isothiocyanate (Boehringer Mannheim).

Techniques: Clone Assay